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rmopn  (R&D Systems)


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    Structured Review

    R&D Systems rmopn
    Rmopn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rmopn/product/R&D Systems
    Average 93 stars, based on 102 article reviews
    rmopn - by Bioz Stars, 2026-03
    93/100 stars

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    93
    MedChemExpress recombinant mouse opn rmopn protein
    <t>rmOPN</t> was supplemented after <t>OPN</t> silencing, and the protective effects in MH-s cells treated with LPS were reversed. ( A ) ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells. ( B ) The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. ( C ) Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; <t>recombinant</t> mouse OPN, rmOPN; interleukin, IL; tumour necrosis factor, TNF; NOD-, LRR- and pyrin domain-containing 3, NLRP3; Gasdermin D, GSDMD; apoptosis-associated speck-like protein containing a CARD, ASC; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; enzyme-linked immunosorbent assay, ELISA; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR
    Recombinant Mouse Opn Rmopn Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse opn rmopn protein/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    recombinant mouse opn rmopn protein - by Bioz Stars, 2026-03
    93/100 stars
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    93
    R&D Systems rmopn
    <t>rmOPN</t> was supplemented after <t>OPN</t> silencing, and the protective effects in MH-s cells treated with LPS were reversed. ( A ) ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells. ( B ) The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. ( C ) Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; <t>recombinant</t> mouse OPN, rmOPN; interleukin, IL; tumour necrosis factor, TNF; NOD-, LRR- and pyrin domain-containing 3, NLRP3; Gasdermin D, GSDMD; apoptosis-associated speck-like protein containing a CARD, ASC; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; enzyme-linked immunosorbent assay, ELISA; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR
    Rmopn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rmopn/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    rmopn - by Bioz Stars, 2026-03
    93/100 stars
      Buy from Supplier

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    rmOPN was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. ( A ) ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells. ( B ) The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. ( C ) Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; recombinant mouse OPN, rmOPN; interleukin, IL; tumour necrosis factor, TNF; NOD-, LRR- and pyrin domain-containing 3, NLRP3; Gasdermin D, GSDMD; apoptosis-associated speck-like protein containing a CARD, ASC; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; enzyme-linked immunosorbent assay, ELISA; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR

    Journal: Journal of Inflammation (London, England)

    Article Title: The injury effect of osteopontin in sepsis-associated lung injury

    doi: 10.1186/s12950-025-00430-4

    Figure Lengend Snippet: rmOPN was supplemented after OPN silencing, and the protective effects in MH-s cells treated with LPS were reversed. ( A ) ELISAs were used to detected the concentration of IL-6 and TNF-α in cell supernatant of MH-s cells. ( B ) The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. ( C ) Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; recombinant mouse OPN, rmOPN; interleukin, IL; tumour necrosis factor, TNF; NOD-, LRR- and pyrin domain-containing 3, NLRP3; Gasdermin D, GSDMD; apoptosis-associated speck-like protein containing a CARD, ASC; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; enzyme-linked immunosorbent assay, ELISA; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR

    Article Snippet: The cells were then divided into two groups: the experimental group, which was treated with 200 ng/mL of recombinant mouse OPN (rmOPN) protein (MCE, Osteopontin, HY-P78358, USA) for 30 min, and the control group, which received no such treatment.

    Techniques: Concentration Assay, Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction